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101.
《Journal of Cardiovascular Computed Tomography》2020,14(6):466-470
The 15th Society of Cardiovascular Computed Tomography (SCCT) annual scientific meeting (ASM) welcomed 770 digital attendees from 44 countries, over 2 days, with a program that included 30 sessions across three simultaneously streaming channels, 10 exhibitors and a diverse range of scientific abstracts. In addition, #SCCT2020 generated >5900 tweets from nearly 700 engaged social media participants resulting in an estimated 38 million digital impressions and becoming #1 trending medical meeting in social media in the world during the meeting time period. This article summarizes the many themes and topics of presentation and discussion in this meeting, and the many technical advances that are likely to impact future clinical practice in cardiovascular computed tomography. 相似文献
102.
103.
《Seminars in Fetal & Neonatal Medicine》2020,25(5):101121
The current standard approach to manage circulatory insufficiency is inappropriately simple and clear: respond to low blood pressure to achieve higher values. However, the evidence for this is limited affecting all steps within the process: assessment, decision making, therapeutic options, and treatment effects. We have to overcome the ‘one size fits all’ approach and respect the dynamic physiologic transition from fetal to neonatal life in the context of complex underlying conditions. Caregivers need to individualize their approaches to individual circumstances. This paper will review various clinical scenarios, including managing transitional low blood pressure, to circulatory impairment involving different pathologies such as hypoxia-ischemia and sepsis. We will highlight the current evidence and set potential goals for future development in these areas. We hope to encourage caregivers to question the current standards and to support urgently needed research in this overlooked but crucial field of neonatal intensive care. 相似文献
104.
目的 通过对广西不同地区福寿螺的COⅠ基因进行分析,以了解广西福寿螺种群的遗传多态性。方法 从南宁市横县、桂林市全州县和荔浦县、贺州市富川县、百色市田林县和崇左市凭祥县共计6个县区采集福寿螺样本,提取DNA并进行COⅠ基因的PCR扩增及测序。运用MAGE 7.0将本研究获得的单倍型与GenBank中的福寿螺单倍型使用邻接法构建系统进化树,并计算个体间的遗传距离,分析其遗传多样性。结果 COⅠ基因长度为493 bp,从11个样本鉴定出2种单倍型:单倍型1(Haplotype 1)和单倍型2(Haplotype 2)。其中9个样本为Haplotype 1,分别来源于南宁市横县(2个)、贺州市富川县(1个)、桂林市荔浦县(1个)、百色市田林县(2个)、崇左市凭祥县(3个),该单倍型与小管福寿螺遗传距离最近,为0.047;2个样本为Haplotype 2,分别源自为南宁市横县和桂林市全州,与孤岛福寿螺遗传距离最近,为0.062。根据上述条件所构建的进化树形成了7个分支,分别为P. canaliculata、P. camena、P. insularum、P. paludosa、P. diffusa、P. haustrum、和外群Pilaconica。Haplotype 1与来自美国夏威夷 (GenBank登录号 EU 523129)的P. canaliculata组成一个分支,Haplotype 2与来自巴西(GenBank登录号EF514942)的P. insularum组成一个分支,且置信值均在98%以上。结论 初步判断广西地区存在小管福寿螺与孤岛福寿螺两种类型的福寿螺,其种群内没有发现遗传分化。 相似文献
105.
Thais Basili Higinio Dopeso Sarah H. Kim Lorenzo Ferrando Fresia Pareja Arnaud Da Cruz Paula Edaise M. da Silva Anthe Stylianou Ana Maroldi Caterina Marchiò Brian P. Rubin Mauro Papotti Britta Weigelt Carlos Gil Moreira Ferreira José Roberto Lapa e Silva Jorge S. Reis-Filho 《International journal of cancer. Journal international du cancer》2020,147(8):2253-2264
Hyalinizing trabecular tumors of the thyroid are rare and mostly benign epithelial neoplasms of follicular cell origin, which have recently been shown to be underpinned by the PAX8-GLIS3 fusion gene. In our study, we sought to investigate the potential oncogenic mechanisms of the PAX8-GLIS3 fusion gene. Forced expression of PAX8-GLIS3 was found to increase proliferation, clonogenic potential and migration of human nonmalignant thyroid (Nthy-ori 3-1) and embryonic kidney (HEK-293) cells. Moreover, in xenografts, Nthy-ori 3-1 PAX8-GLIS3 expressing cells generated significantly larger and more proliferative tumors compared to controls. These oncogenic effects were found to be mediated through activation of the Sonic Hedgehog (SHH) pathway. Targeting of smoothened (SMO), a key protein in the SHH pathway, using the small molecule inhibitor Cyclopamine partially reversed the increased proliferation, colony formation and migration in PAX8-GLIS3 expressing cells. Our data demonstrate that the oncogenic effects of the PAX8-GLIS3 fusion gene are, at least in part, due to an increased activation of the SHH pathway. 相似文献
106.
Noriaki Yoshida Hiroaki Miyoshi Fumiko Arakawa Kazutaka Nakashima Keisuke Kawamoto Masao Seto Koichi Ohshima 《Hematological oncology》2020,38(5):673-679
Follicular lymphoma (FL) is a germinal center-derived B-cell lymphoma that is known to proliferate in the intrafollicular region. However, lymphoma cells can be identified in the extrafollicular regions, which are related to disease dissemination. We purified the intrafollicular and extrafollicular regions of FL cells by laser microdissection and conducted microarray analysis in order to characterize the gene expression profiles of FL cells from both regions. BCL2 and genes of germinal B-cell markers clearly separated intrafollicular and extrafollicular regions of reactive follicular hyperplasia, suggesting the adequacy of the current analysis. In FL cases, cytokine-related genes were significantly enriched in extrafollicular regions compared with those in the intrafollicular regions. In intrafollicular regions of FL, cell-cycle–related genes were enriched. We found that the FL cells in the extrafollicular region more strongly expressed IL3RA and CXCL12 than those of intrafollicular regions. The cytokines might be also derived from stroma cells in the extrafollicular regions, which may initiate activation and migration of the tumor cells to this region. Our results suggest that FL cell interaction with surrounding stroma cells plays an important role in the pathophysiology of FL and that such interactions should be a good target for therapy. 相似文献
107.
108.
乳腺癌是一类具有异质性的肿瘤,不同患者的治疗方法和疗效都不相同。尽管目前仍在努力为激素受体(hormone receptor,HR)阳性(+)、人表皮生长因子受体2(human epidermal growth factor receptor 2,HER-2)阴性(-)、淋巴结(axillary lymph node,ALN)阴性(-)的早期乳腺癌患者寻找合适的治疗方法,但其术后是否需要化疗仍然是肿瘤科医生面临的一个难题。以往治疗主要依赖于经典的组织病理学和免疫组织化学技术,随着精准医疗时代的到来,我们需要更定量的诊断方法和合理的个体化治疗。虽然化疗可降低疾病复发风险并提高生存率,但它带来的不良反应事件会降低患者的生活质量,尤其低复发风险(recurrence risk,RS)有可能超过化疗益处。21基因检测不仅可以预测这类早期乳腺癌化疗疗效及评估预后,还可提供精准的个体化治疗方案指导用药,为患者增添信心。本文就乳腺癌21基因检测的研究进展进行综述。 相似文献
109.
目的探讨ABCG5基因突变所致的植物固醇血症的临床及基因变异特征。方法回顾分析1例ABCG5基因突变致植物固醇血症患儿的临床资料。结果 1岁3个月男性幼儿,约4月龄时腕、踝关节皮肤褶皱处开始出现线状黄瘤,后渐加重。血固醇谱检查示菜油固醇、二氢胆固醇明显升高。全外显子检测示ABCG5基因c.904+1(IVS7)GA剪切位点突变和c.-76(exonl)CT非编码区突变。经严格控制植物固醇摄入、少量限制动物固醇摄入以及口服消胆胺治疗40天后,复查患儿血固醇谱,植物固醇水平较前明显降低。结论 ABCG5变异可致植物固醇血症,及时诊断以及药物和饮食控制可改善预后。 相似文献
110.
目的探讨先天性肾性尿崩症的临床特点、基因诊断及治疗。方法回顾分析2例先天性肾性尿崩症患儿的临床资料。结果 2例男性患儿分别为5岁和3岁2个月,均以多饮多尿、生长迟缓为主要表现。经禁水-加压素试验证实为持续低比重尿。尿崩症相关基因检测发现,例1患儿精氨酸加压素受体2(AVPR2)基因外显子2杂合错义突变c.650CT(p.P217L),且为新发变异。例2患儿AVPR2基因外显子1及外显子2缺失,亦为新发变异,其母亲为携带者,父亲AVPR2基因未见异常变异。对新发的变异位点通过Mutation-taster及Polyphen2软件预测为致病性变异。2例患儿口服氢氯噻嗪联合吲哚美辛治疗1年,尿量及夜尿减少,无电解质紊乱及肾功能受损等。结论 AVPR2基因为先天性肾性尿崩症的主要致病基因,发现2种国内外未见报道的新变异位点。 相似文献